Lunch vendor seminar, November 4, 2009 (12:45–14:00)
Rapid Tests for Allergens and Mycotoxins
Sponsored by 
Rapid Detection of Allergens – New Approach of LFD Technology
Dr. Sigrid Haas-Lauterbach1, Dr. Ulrike Immer1,, Dr. Karl Schmitt2,
1 R-Biopharm AG, Germany
2 Bioavid Diagnostics GmbH & Co. KG, Germany
The key point in allergen and gluten analysis is the availability of efficient and sensitive methods for the detection of the targets from both unprocessed and heat-processed food samples including an all purpose extraction procedure. To ensure an adequate labeling of allergenic food compounds for consumer safety, and to enable enforcement of the allergen regulations in place, different analytical methods have been developed. Most of them are antibody based like ELISA (enzyme linked immunosorbent assay) which has considerable applications for food and cereal analysis.
Hidden allergens caused by cross contamination are a not discernible risk for allergic consumers. This potential risk is not covered by any labeling regulation. Therefore, industries often use precautionary labeling, which is not of help for the allergenic consumer. To minimize this kind of allergen labeling of products, appropriate allergen sanitation procedures are required. For monitoring the efficiency of cleaning procedures of the environment and shared equipment, the lateral flow devices (LFD) have the needed features like sensitivity, specificity, easy handling and rapid evaluation. Such tests are available for a number of individual allergens. They allow qualitative detection of traces of allergens in about 15 minutes of total assay time and do not require any equipment for evaluation. While the method of choice in sanitation control is swabbing, with a little more sample preparation these tests can be used also for qualitative detection of allergen residues in raw materials or even processed food.
RIDA®QUICK Mycotoxin Lateral Flow Assays – A New Approach for Mycotoxin Analysis
Dr. Walter Luebbe, Dr. Michael Maettner, Johannes Winkle, Dr. Martin Mehl, Ronald Niemeijer
R-Biopharm AG, Darmstadt, Germany, info@r-biopharm.de
The RIDA®QUICK Mycotoxin product line is a novel immunochromatographic lateral flow format for the detection of aflatoxin, and deoxynivalenol from grain and cereals. The inverted competitive assay format is based on the directly proportional reaction of the target molecule with specific gold-labelled antibodies. This means as soon as mycotoxins above detection level are presented, a result line occurs. A control line assures the validity of the test run. After extraction and sedimentation the sample is applied to the test membrane. Depending on incubation time, between 2 and 16 minutes, different concentrations of applied mycotoxins can be determined semi-quantitative visually. For documentation purposes the reaction can be stopped and the sticks can be stored for several months.
Increasing demands on quality control and consumer protection requires more and more fast and reliable testing of raw materials and processed food. Contemporary assessment of raw materials guarantee a cost and time efficient distribution and production. The new RIDA®QUICK DON and RIDA®QUICK Aflatoxin, optional combined with the RIDA®QUICK SCAN, fulfils the requirements of modern mycotoxin analysis.
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